A recent article from the Reineke Group published in Accounts of Chemical Research, entitled “Nonviral Gene Delivery with Cationic Glycopolymers” and published online on April 17, 2019, has achieved the status of being one of the top three most downloaded articles in the journal for the month of April. This article summarizes the group’s 16 years of research in the field of DNA delivery. Additionally, this Account was selected for ACS Editors’ Choice, making the article open access and freely available for anyone to read.